Ginkgo biloba Extract: A Preliminary Study on Its Radiomitigative Potential Using the Conventional Dicentric Chromosome Assay: Extrato de Ginkgo biloba: Um estudo preliminar sobre seu potencial radiomitigador usando o ensaio convencional de cromossomo dicêntrico

Julio Cesar Gomes da Silva; CAIO C. V. B. G; MENDES M. E.; LOPES I. M. S. S.; MELO A. M. M. A; LIMA F. F.

doi:10.15392/2319-0612.2025.2910

Authors

Julio Cesar Gomes da Silva Federal University of Pernambuco
CAIO C. V. B. G Universidade Federal de Pernambuco UFPE , Federal University of Pernambuco
MENDES M. E. Centro Regional de Ciências Nucleares do Nordeste, CRCN-NE, Recife, PE.
LOPES I. M. S. S. Universidade Federal de Pernambuco UFPE , Federal University of Pernambuco
MELO A. M. M. A Universidade Federal de Pernambuco UFPE
LIMA F. F. Centro Regional de Ciências Nucleares do Nordeste, CRCN-NE

DOI:

https://doi.org/10.15392/2319-0612.2025.2910

Keywords:

Ginkgo biloba, Ionizing radiation, cytogenetic dosimetry, dicentric

Abstract

Radioprotectors and radiomitigators can be substances of natural or synthetic origin that, once administered to the human body, are capable of preventing, protecting against, or reducing damage caused by radiation. This study aimed to assess the radiomitigating potential of Ginkgo biloba extract by examining the frequency of dicentric chromosomes in irradiated human lymphocytes. Blood samples were obtained from a healthy volunteer. One sample was left in the laboratory as a control, and the others were irradiated in a ⁶⁰Co source at a dose of 2 Gy and incubated in test concentrations of Ginkgo biloba extract (0.025 and 0.05 μg/ml) according to a standard protocol. Complete metaphases were analyzed, and the frequencies of alterations were statistically evaluated to verify the radiomitigating potential of Ginkgo biloba extract. The ANOVA and Tukey tests revealed a statistically significant difference between the groups treated and untreated with Ginkgo biloba exposed to 2 Gy of radiation (p < 0.05), indicating that both concentrations tested were able to reduce the damage. In this in vitro study, Ginkgo biloba extract was able to reduce the frequency of dicentric chromosomes in irradiated human lymphocytes, with a concentration of 0.025 μg/ml standing out.

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Author Biographies

CAIO C. V. B. G, Universidade Federal de Pernambuco UFPE, Federal University of Pernambuco

Postgraduate Program in Biological Sciences, PPGCB
MENDES M. E., Centro Regional de Ciências Nucleares do Nordeste, CRCN-NE, Recife, PE.

Biological Dosimetry Laboratory
LOPES I. M. S. S., Universidade Federal de Pernambuco UFPE, Federal University of Pernambuco

Postgraduate Program in Morphotechnology, PPGM
MELO A. M. M. A, Universidade Federal de Pernambuco UFPE

Department of Biophysics and Radiobiology and Postgraduate Program in Energy and Nuclear Technologies, PROTEN,
LIMA F. F., Centro Regional de Ciências Nucleares do Nordeste, CRCN-NE

Biological Dosimetry Laboratory

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