Dose-effect calibration curve for high X-ray doses using the Calyculin-A chromosome premature condensation assay

Fabio-Andrés Chaves-Campos; Fernando Ortíz-Morales; Ricardo Mejías-Gamboa; Jorge Ernesto González-Mesa; Omar García-Lima; Maria Paula Rodríguez-Valerio; Walter Vargas-Segura; Anthony Cordero-Ramírez

doi:10.15392/2319-0612.2024.2422

Autores/as

Fabio-Andrés Chaves-Campos Health Research Institute, University of Costa Rica. https://orcid.org/0000-0003-3066-5495
Fernando Ortíz-Morales Health Research Institute, University of Costa Rica. San José, Costa Rica. , , https://orcid.org/0000-0002-9120-6308
Ricardo Mejías-Gamboa Health Research Institute, University of Costa Rica. San José, Costa Rica , ,
Jorge Ernesto González-Mesa Centro de Protección e Higiene de las Radiaciones (CPHR), Habana, Cuba. https://orcid.org/0000-0001-7221-4604 (no autenticado)
Omar García-Lima Centro de Protección e Higiene de las Radiaciones (CPHR), Habana, Cuba https://orcid.org/0000-0002-0712-9734 (no autenticado)
Maria Paula Rodríguez-Valerio School of Health Technologies, University of Costa Rica. San José, Costa Rica , ,
Walter Vargas-Segura Physics Department. Costa Rican Institute of Technology, ITCR. Cartago, Costa Rica. , ,
Anthony Cordero-Ramírez Research Center in Materials Science and Engineering (CICIMA), University of Costa Rica, San José, Costa Rica , ,

DOI:

https://doi.org/10.15392/2319-0612.2024.2422

Palabras clave:

aberraciones citogenéticas, biodosimetría, biología de las radiaciones, Caliculina-A, protección radiológica

Resumen

Propósito: este artículo muestra los resultados del Laboratorio de Citogenética del Instituto de Investigación Sanitaria (INISA) para desarrollar una curva de calibración dosis-efecto con el ensayo de condensación prematura por inducción química de caliculina-A para estimar altas dosis de exposición a rayos X. Métodos: para crear la curva de calibración, se expuso muestras de sangre periférica in vitro de dos participantes (una mujer y un hombre) en seis puntos de dosis diferentes que oscilaban entre 0 y 17,5 Gy de rayos X. La sangre irradiada se cultivó durante 48 horas según los protocolos internacionales y se registraron los anillos cromosómicos resultantes. Se utilizó el programa BioDoseTools para calcular los coeficientes de la curva de calibración. Resultados: Los coeficientes de la curva son α 0,028±0,001 y C: 0,001±0,001. Estos coeficientes tienen valores similares a los reportados internacionalmente. La curva se validó calculando una dosis desconocida expuesta a 6 Gy; la dosis estimada fue de 5,651 ± 0,636 Gy, sin diferencias estadísticamente significativas entre la dosis administrada y la dosis estimada. Conclusiones: El Servicio de Dosimetría Biológica del INISA puede utilizar la curva obtenida para estimar dosis absorbidas en casos de sospecha de sobreexposición a altas dosis de rayos X.

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