Gamma radiation in association with moderate training reduce the effects of asthma in mice

Nayara Alves Severo; Cilene Souza Barreto; Maria de Lourdes Meirelles Noviello; João Aguiar Nogueira Batista; Rosiane Resende Leite; Arno Heeren de Oliveria

doi:10.15392/bjrs.v10i3.2068

Autores

Nayara Alves Severo Departamento de Ciências da Saúde - Universidade Estadual de Santa Cruz, BA, Brasil ,
Cilene Souza Barreto Departamento de Ciências da Saúde - Universidade Estadual de Santa Cruz, BA, Brasil
Maria de Lourdes Meirelles Noviello Laboratório de Patologia e Imunologia – Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brasil https://orcid.org/0000-0001-5831-5054 (não autenticado)
João Aguiar Nogueira Batista Laboratório de Patologia e Imunologia – Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brasil
Rosiane Resende Leite Centro Federal de Educação Tecnológica de Minas Gerais, MG, Brasil
Arno Heeren de Oliveria Departamento de Engenharia Nuclear, Universidade Federal de Minas Gerais ,MG, Brasil https://orcid.org/0000-0001-7783-3041 (não autenticado)

DOI:

https://doi.org/10.15392/bjrs.v10i3.2068

Palavras-chave:

Efeitos da radiação, Sistema imunológico, Análise histológica

Resumo

A radiação ionizante tem sido utilizada para o tratamento de várias doenças há mais de um século, incluindo doenças inflamatórias crônicas e câncer. As relações entre radiação e asma são contraditórias; enquanto alguns autores associam a exposição à radiação com o desenvolvimento da doença, outros relatam uma atenuação da asma em resposta à radiação. A asma é uma doença inflamatória crônica e representa um problema de saúde pública mundial com elevado número de óbitos. No presente estudo, realizamos uma investigação dos efeitos da radiação com 10 doses de 0,5Gy de Co60 e/ou treinamento pulmonar moderado de camundongos com asma induzida por ovalbumina. Para tanto, comparamos seis grupos experimentais de camundongos: 1-Soro fisiológico (não irradiado, sedentário e salino); 2- RI (irradiado e sedentário); 3- OVA (não irradiado, sedentário e asmático); 4- OVA+IR (irradiados, asmáticos e sedentários); 5- OVA+IR+MT (irradiado, asma e treinamento moderado -TM); 6- OVA+MT (asma e treinamento moderado). Os resultados indicam que a radiação e o treinamento moderado reduziram significativamente os parâmetros inflamatórios tanto nas células BALF quanto na produção de muco, atenuando assim os sintomas da asma

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Referências

J.Bernier, E.J. Hall and A. Giaccia, “Radiation oncology: a century of achievements”, Nature Reviews Cancer, vol. 4, no. 9, pp. 737-747, 2004.

G. M. Ross, ‘Induction of cell death by radiotherapy’, Endocrine-related cancer, vol. 6, no. 1, pp. 41-44, 1999.

S. Demaria and S.C. Formenti “Radiation as an immunological adjuvant: current evidence on dose and fractionation”. Frontiers in oncology, vol. 2, pp. 153, 2012.

D.S CHEN and I. MELLMAN, “Oncology meets immunology: the cancer-immunity cycle”, Immunity, vol. 39, no. 1, pp. 1-10, 2013.

B.Park, C.Yee and K. M. Lee, “ The effect of radiation on the immune response to cancers”, International journal of molecular sciences, Switzerland, v. 15, n. 1, p. 927-943, 2014.

T. Bhattacharyya, K. Purushothaman, S. S. V. Puthiyottil, A.Bhattacharjee, and G. Muttah, “Immunological interactions in radiotherapy—opening a new window of opportunity”, Annals of Translational Medicine, vol. 4, no. 3, 2016.

J. LINDEN, “Optimizing radiation for cancer immunotherapy”, Translational Cancer Research, vol. 5, no. 2, pp. 191-193, 2016.

I.V. Mavragani, D. A. Laskaratou, B. Frey, S. M. Candéias, U.S Gaipl K. Lumniczky and A. G. Georgakilas, “Key mechanisms involved in ionizing radiation-induced systemic effects”. A current review. Toxicology Research, vol. 5, no. 1, p. 12-33, 2016.

S.Z. Liu, “Nonlinear dose-response relationship in the immune system following exposure to ionizing radiation: mechanisms and implications”. Nonlinearity in biology, toxicology, vol. 1, no. 1, 2003.

L. F. Rödel, L. Keilholzand, M, .Herrmann, R.Sauer and G. Hildebrandt, “Radiobiological mechanisms in inflammatory diseases of low-dose radiation therapy”. International journal of radiation biology, vol. 83, no. 6, p. 357-366, 2007.

M.Arenas, S. Sabater, V. Hernández, A. Rovirosa, P.C. Lara, A. Biete and J. Panés, “Anti-inflammatory effects of low-dose radiotherapy, ” Strahlentherapie und Onkologie, vol. 188, no. 11, pp. 975-981, 2012

H. Nakatsukasa, M. Tsukimoto, A. Tokunaga, and S. Kojima, “ Repeated gamma irradiation attenuates collagen-induced arthritis via up-regulation of regulatory T cells but not by damaging lymphocytes directly”. Radiation research, vol. 174, no. 3, pp. 313-324, 2010.

E.J Calabrese and G. Dhawan, “The role of x-rays in the treatment of gas gangrene: a historical assessment.” Dose Response, vol. 10, no. 4, pp. 12-016, 2012.

E. J.Calabrese, G. Dhawan and R. Kapoor, “The Use of X Rays in the Treatment of Bronchial Asthma: A Historical Assessment”. Radiation research, vol. 184, no. 2, pp. 180-192, 2015.

S.P. Fang, F. Tago, T. Tanaka, N. Simura, Y. Muto, R. Goto and S. Kojima, “Repeated irradiations with gamma-rays at a Dose of 0.5 Gy may exacerbate asthma”. Journal of radiation research, vol. 46, no. 2, pp. 151-156, 2005.

B.S. Park, G.U. Hong and J.Y. Ro,” Foxp3+-Treg cells enhanced by repeated low-dose gamma-irradiation attenuate ovalbumin-induced allergic asthma in mice,” Radiation research, vol. 179, no. 5, pp. 570-583, 2013.

S.J. Galli, M. Tsai and A.M. Piliponsky, “The development of allergic inflammation”, Nature, vol. 454, no. 7203, p. 445-454, 2008.

B. N. Lambrecht and H. Hammad. “The immunology of asthma”, Nature immunology, vol. 16, no. 1, p. 45-56, 2015.

Gina (Global Initiative for Asthma). Global strategy for asthma management and prevention. 2016. http://ginasthma.org/wp-content/uploads/2016/04/GINA-2016-main-report_tracked.pdf.

D. H. Broide, “Molecular and cellular mechanisms of allergic disease”, Journal of allergy and clinical immunology, vol. 108, no. 2, pp. S65-S71, 2001.

D.K. Agrawal and Z. Shao, “Pathogenesis of allergic airway inflammation”. Current Allergy Asthma Reports, vol.10, no 39, 2010.

S. Croisant, “Epidemiology of asthma: prevalence and burden of disease”. Heterogeneity in Asthma, pp. 17-29 , 2014.

T.Aikawa, S. Shimura, H. Sasaki, M. Ebina and T. Takishima, “Marked goblet cell hyperplasia with mucus accumulation in the airways of patients who died of severe acute asthma attack”. Chest Journal, vol. 101, no. 4, pp. 916-921, 1992.

L. M. Kuyper, P. D. Paré, J. C. Hogg, R. K. Lambert, D. Ionescu, R.Woods and T. R. Bai, “Characterization of air- way plugging in fatal asthma,” The American journal of medicine, vol. 115, no. 1, pp. 6-11, 2003.

E. Ortega, “The “bioregulatory effect of exercise” on the innate/inflammatory responses. Journal of physiology and biochemistry, pp. 1-9, 2016.

N. P. Walsh, M.Gleeson, D. B. Pyne et al., “Position Statement Part two: Maintaining immune health,” Exerc Immunol Rev, vol.17, pp.64-103, 2011.

D.Menicucci, A. Piarulli, F. Mastorci et al., " Interactions between immune, stress-related hormonal and cardiovascular systems following strenuous physical exercise.” Archives Italiennes de Biologie, vol. 151, no. 3, pp. 126-36, 2013.

P. Q.Llopiz and M. R. García-Galbis, “Control glucémico a través del ejercicio físico en pacientes con diabetes mellitus tipo 2; revisión sistemática”. Nutrición Hospitalaria, vol. 31, no. no4, p. 1465-1472, 2015.

G. I Lancaster and M.A. Febbraio, “The immunomodulating role of exercise in metabolic disease,”Trends in immunology, vol. 35, no. 6, p. 262-269, 2014.

E.C. Gomes and G. Florida-James, “Exercise and the Immune System”. Environmental Influences on the Immune System, pp. 127-152. 2016.

L. M. Knippels, A. H. Penninks, J. J. Smit and G. F. Houben, “Immune-mediated effects upon oral challenge of ovalbumin-sensitized Brown Norway rats: further characterization of a rat food allergy model”. Toxicology and applied pharmacology,

vol. 156, no. 3, p. 161-169, 1999.

M. O. Meneguello, L. F. B. Rosa, “Efeito da restrição calórica e do exercício aeróbio em linfócitos e macrófagos de ratos envelhecidos”. Revista Paulista de Educação Física, vol. 16, pp. 16-26, 2002.

H. S. Campos, “Asthma: its origins, inflammatory mechanisms and the role of the corticosteroid”, Rev Bras Pneumol Sanit, vol. 15, no. 1, p. 47-60, 2007..

T. Ohtomo, O. Kaminuma, J. Yamada et al., “Eosinophils are required for the induction of bronchial hyperresponsiveness in a Th transfer model of BALB/c background”. International archives of allergy and immunology, vol. 152, no. Suppl. 1, pp. 79-82, 2010.

V. Cottin, R. Frognier, H. Monnot et al. “Chronic eosinophilic pneumonia after radiation therapy for breast cancer”. European Respiratory Journa, vol. 23, no. 1, pp. 9-13, 2004

Q. Hamid and M. Tulic, “Immunobiology of asthma”. Annual Review of Physiology, vol. 71, pp. 489-507, 2009.

L.George and C. E. Brightling. “ Eosinophilic airway inflammation: role in asthma and chronic obstructive pulmonary disease”. Therapeutic advances in chronic disease, vol.7, pp. 34 – 51, 2015

S.H. KIM, “Airway epithelial cells in airway inflammation and remodeling in asthma”. Allergy, Asthma & Respiratory Disease, vol. 4, no. 2, p. 82-90, 2016.

J.S. Kim, Y. Son, M.J. Bae et al., “Continuous Exposure to Low- Dose-Rate Gamma Irradiation Reduces Airway Inflammation in Ovalbumin-Induced Asthma”. PloS one, vol. 10, no. 11, p. e0143403, 2015.

J. A. Boyce, “The role of mast cells in asthma”. Prostaglandins, leukotrienes and essential fatty acids, vol. 69, no. 2, pp. 195-205, 2003.

R. Riekki, I. T. Harvima, A. Jukkola, J. Risteli, and A. Oikarinen, “The production of collagen and the activity of mast‐cell chymase increase in human skin after irradiation therapy,” Experimental dermatology, vol. 13, no. 6, p. 364-371, 2004.

P. H. Hart, “Regulation of the inflammatory response in asthma by mast cell products”, Immunology and Cell Biology, vol. 79, no. 2, p. 149-153, 2001.

H. M. Joo, S. Y. Nam, K. H. Yang, et al. “ The effects of low-dose ionizing radiation in the activated rat basophilic leukemia (RBL-2H3) mast cells”,. Journal of Biological Chemistry, vol. 287, no. 33, p. 27789-27795, 2012.

W. D. M. Cruvinel, D.Mesquita Júnior, J. A. P. Araújo, et al. “Immune system: Part I. Fundamentals of innate immunity with emphasis on molecular and cellular mechanisms of inflammatory response,” Revista brasileira de reumatologia, vol. 50, no. 4, p. 434-447, 2010.

E. Morcillo, J. Cortijo, “Mucus and MUC in asthma”. Current opinion in pulmonary medicine, vol. 12, no. 1, p. 1-6, 2006.

A. da C. Lana, C. A, Paulino and I. D. Gonçalves, “Efeitos dos exercícios físicos sobre o edema inflamatório agudo em ratos Wistar”. Revista Brasileira de Medicina do Esporte, vol. 14, no. 1, pp. 33-37, 2008.

B. K. Pedersen and L. Hoffman-Goetz. “Exercise and the immune system: regulation, integration, and adaptation”, Physiological reviews, vol. 80, no. 3, p. 1055-1081, 2000.

D. P. S. Lopes, I. P. R Muniz and R. A. A Da Silva, “Intensidade de Exercício Físico e Imunomodulação: Impactos em Infecções das Vias Aéreas”, Saúde e Pesquisa, vol. 9, no. 1, p. 175-186, 2016

A. H. Van Craenenbroeck, K.Van Ackeren, V. Y. Hoymans et al. “Acute Exercise-Induced Response of Monocyte Subtypes in Chronic Heart and Renal Failure.” Mediators of inflammation, vol. 2014, pp. 1-11, 2014.

E. J Dhurandhar, S. W. Keith, “The aetiology of obesity beyond eating more and exercising less.” Best Pract Res Clin Gastroenterol, vol. 28, no. 4, pp. 533-544, 2014.

S. N. Culos-Reed, J. L. Robinson, H. Lau, K. O Connor and M. R. Keats, “Benefits of a physical for activity intervention men with prostate cancer”, Journal of sport and exercise psychology, vol. 29, no. 1, pp. 118, 2007.

C. M. Schneider, C. C. Hsieh, L. K. Sprod, S. D.Carter and R. Hayward, “Effects of supervised exercise training on cardiopulmonary function and fatigue in breast cancer survivors during and after treatment”. Cancer, vol. 110, no. 4, p. 918-925, 2007.

A.S. Betof, M.W. Dewhirst, L. W. “Jones Effects and potential mechanisms of exercise training on cancer progression: a translational perspective”. Brain, behavior, and immunity, vol. 30, pp. S75-S87, 2013.

A. Edsfeldt, H. Grufman, G. Asciuttoet al., “Circulating cytokines reflect the expression of pro-inflammatory cytokines in atherosclerotic plaques”. Atherosclerosis, Switzerland, v. 241, n. 2, p. 443-449, 2015.

F. Rödel, B. Frey, U. Gaipl, et al., “Modulation of inflammatory immune reactions by low-dose ionizing radiation: molecular mechanisms and clinical application”. Current medicinal chemistry, vol. 19, no. 12, pp. 1741-1750, 2012.

G. Hildebrandt, A. Radlingmayr, S. et al., “Low-dose radiotherapy (LD-RT) and the modulation of iNOS expression in adjuvant-in¬duced arthritis in rats”, International journal of radiation biology, vo. 79, pp. 993–1001, 2003.

M. Arenas, F. Gil, M. Gironella, V. Hernandez et al. “ Anti-inflammatory effects of low-dose radiotherapy in an experimental model of systemic inflammation in mice”. International Journal of Radiation Oncology* Biology* Physics, vol. 66, no. 2, pp. 560-567, 2006.

M. Tsukimoto, T. Homma, Y. Mutou and S. Kojima “0.5 Gy gamma radiation suppresses production of TNF-alpha through up-regulation of MKP-1 in mouse macrophage RAW264.7 cells” , Radiat. Res. Vol. 171 , pp.219–224, 2009.

Chew, M. T, Eman, D. Mayeen, U. K., Bleddyn, J., Andrew, N., Bradley, D. A. “Low radiation dose to treat pneumonia and other inflammations”. The British Journal of RadiologyVol. 94, No. 1124, 2021.

C. Wenshu, X. Xiuling, B. Lang, M.T Padilla, L. Shuguang; C.S Tellez, M.G Katherine, J.A. Wilder, S.A. Belinsky, B. R. Scott, and Lin, Y. “Low-dose gamma-irradiation inhibits IL-6 secretion from human lung fibroblasts that promotes bronchial epithelial cell transformation by cigarette-smoke carcinogen”. Carcinogenesis vol.33 no.7 pp.1368–1374, 2012

C. L. Gallic, Y. Phalente, L. Manens, I. Dublineau, M. Benderitter, Y. Gueguen, S.Lehoux, T. G. Ebrahimian. “Chronic Internal Exposure to Low Dose 137Cs Induces Positive Impact on the Stability of Atherosclerotic Plaques by Reducing Inflammation in ApoE-/- Mice”. PLOS ONE | June 5, 2015.