In Vivo distribution dynamics of Gold Nanoparticles: A quantitative analysis

Angélica  Barbezan; Wilmmer Alexander Rosero; Luis Fernando Almeida; Maria Eduarda Rigo; Fabio Fernando Silva; Maria Elisa  Rostelato

doi:10.15392/2319-0612.2024.2602

Autores/as

Angélica Barbezan Instituto de Pesquisas Energéticas e Nucleares - IPEN , Instituto de Pesquisas Energéticas e Nucleares (IPEN) https://orcid.org/0000-0001-7615-9091 (no autenticado)
Wilmmer Alexander Rosero Instituto de Pesquisas Energéticas e Nucleares - IPEN
Luis Fernando Almeida Instituto de Pesquisas Energéticas e Nucleares - IPEN
Maria Eduarda Rigo Instituto de Pesquisas Energéticas e Nucleares - IPEN
Fabio Fernando Silva Instituto de Pesquisas Energéticas e Nucleares - IPEN
Maria Elisa Rostelato Instituto de Pesquisas Energéticas e Nucleares - IPEN

DOI:

https://doi.org/10.15392/2319-0612.2024.2602

Palabras clave:

Nanopartículas de Oro (AuNPs), Nanobraquiterapia, Goma Arábiga (GA), Biodistribución

Resumen

Este estudio investiga la biodistribución de nanopartículas de oro funcionalizadas con goma arábiga (AuNPs GA) en ratones Balb/C Nude tras la administración intravenosa. Utilizamos dos regímenes de dosificación (200 µCi y 600 µCi) y analizamos los perfiles de distribución en dos puntos de tiempo (3 y 24 horas). Los resultados mostraron una acumulación significativa de AuNPs GA en el hígado y la vesícula biliar, lo que sugiere la excreción biliar como una posible vía. Se observó una reducción en la actividad hepática después de 24 horas, lo que indica biotransformación o excreción de las nanopartículas. La administración intratumoral presentó un perfil de biodistribución más favorable, con menor acumulación en órganos vitales y mayor concentración en células tumorales, destacando la importancia de la vía de administración. Estos hallazgos son cruciales para optimizar las propiedades de biodistribución de las AuNPs para aplicaciones clínicas, contribuyendo significativamente al avance de la nanomedicina y al desarrollo de terapias dirigidas y seguras

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Biografía del autor/a

Angélica Barbezan, Instituto de Pesquisas Energéticas e Nucleares - IPEN, Instituto de Pesquisas Energéticas e Nucleares (IPEN)

I hold a Bachelor's degree in Biomedicine from the Centro Universitário das Faculdades Metropolitanas Unidas (2007), with specializations in clinical analysis, imaging, and pharmacology. Subsequently, I joined the Institute of Energy and Nuclear Research at the University of São Paulo (IPEN/USP), where I earned my Master’s degree in 2012. My Master’s project focused on comparing the labeling of phosphonates, specifically MDP, EDTMP, and Clodronate, with potential therapeutic applications for patients with bone metastases. This initial experience sparked my interest in the therapeutic applications of nuclear biomedicine, motivating me to delve deeper into research.

Furthering my commitment to research, I pursued and completed my Ph.D. at the same institution in 2017, concentrating on in vitro studies of the genotoxicity and cytotoxicity in hepatic cells due to the formation of 2-Alkylcyclobutanones from the irradiation of fat-containing foods. This work provided me with a deeper understanding of the biological effects of radiation and reinforced my dedication to developing safer and more effective therapies.

Currently, I am part of a specialized team in nanobrachytherapy, focusing on the research and development of radioactive gold-198 nanoparticles for prostate cancer therapy. My work involves conducting biological tests, including both in vitro and in vivo studies, with the aim of contributing to significant advancements in cancer treatment, providing new therapeutic options that can improve patient outcomes and quality of life.

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