Analysis of the coxal fluid of O. brasiliensis tick species by analytic techniques

Cibele Bugno Zamboni; Dalton Nogueira da Silva Giovanni; Simone Michaela Simons

doi:10.15392/2319-0612.2024.2628

Autores/as

Cibele Bugno Zamboni Instituto de Pesquisas Energéticas e Nucleares, IPEN – CNEN/SP São Paulo, São Paulo, Brasil , Instituto de Pesquisas Energéticas e Nucleares, IPEN – CNEN/SP São Paulo, São Paulo, Brasil https://orcid.org/0000-0001-7766-2692 (no autenticado)
Dalton Nogueira da Silva Giovanni Instituto de Pesquisas Energéticas e Nucleares - IPEN-CNEN/SP https://orcid.org/0000-0001-7607-1612 (no autenticado)
Simone Michaela Simons Fundação Butantan: São Paulo, São Paulo, BR https://orcid.org/0000-0003-3390-4535 (no autenticado)

DOI:

https://doi.org/10.15392/2319-0612.2024.2628

Palabras clave:

EDXRF, INAA, Elemental Composition, Coxal Liquid, Ornithodoros brasiliensis

Resumen

The coxal fluid of the tick species Ornithodoros brasiliensis (O. brasiliensis) was subjected to detailed chemical analysis using Instrumental Neutron Activation Analysis (INAA) and Energy Dispersive X-ray Fluorescence (EDXRF). These complementary techniques allowed the identification and quantification of key elements such as sodium (Na), chlorine (Cl), potassium (K), and zinc (Zn), along with others like sulfur (S), phosphorus (P), and iron (Fe). The study demonstrated the consistency between the two analytical methods and revealed significant data about the tick's physiological adaptations related to fluid excretion and blood meal processing. Additionally, the absence of heavy metals in the analyzed samples was confirmed. Understanding the composition of coxal fluid is vital for interpreting metabolic processes and exploring its toxicological potential, given the aggressive nature of O. brasiliensis, known to cause severe health impacts on humans and animals. This research contributes to advancing knowledge about tick biology and supports the development of public health measures against tick-borne hazards.

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