In vivo genotoxicity of 2-alkylcyclobutanones in liver cells from rats fed with irradiated cocoa butter using flow citometry.

Authors

  • Regiane Martins Instituto de pesquisas energéticas e nucleares
  • Daniel Perez Vieira Instituição de pesquisas energéticas e nucleares
  • Luma Ramirez de Carvalho Instituição de pesquisas energéticas e nucleares
  • Angélica Bueno Barbezan Instituição de pesquisas energéticas e nucleares
  • Anna Lucia Casañas Haasis Villavicencio Instituição de pesquisas energéticas e nucleares

DOI:

https://doi.org/10.15392/bjrs.v9i1A.1534

Keywords:

2-Alkylcyclobutanones, Micronucleous test, cocoa butter

Abstract

Food irradiation proves to be an effective technique of eliminating some pathogens from food and this has gained significant attention to its potential for food safety. Since 1990, studies on the toxicological safety of 2-Alkylcyclobutanones have been conducted extensively. 2-Alkylcyclobutanones are unique radiolytic products generated by the radiation-induced breakage  of triglycerides in food, 2-dodecylcyclobutanone (2-dDCB) and 2-tetradecylcyclobutanone (2-tDCB) are the predominant compounds detected . Despite studies showing non-genotoxicity of 2-ACBs (2-Alkylcyclobutanones), the results are conflicting and therefore we continue the studies in order to confirm the compounds  are safety for human health. In vivo micronucleus test were performed to verify the 2-ACBs genotoxic effects in hepatic cells using flow citometry. We used cocoa butter irradiated with 20 kGy. A group with animals  was treated with daily intake of irradiated cocoa butter, synthesized 2-dDCB and 2-tDCB for one month. Hepatic cells were selected for genotoxicity analysis due to the liver importance in the compounds metabolization. The improvement of the analytical techniques is important for the research future since the irradiation process is already consolidated. The results confirmed the safety of the food irradiation process, as they did not indicate the genotoxic potential of the samples.

 

Keywords: 2- Alkylcyclobutanones, Micronucleous test, liver.

Downloads

Download data is not yet available.

Author Biographies

Regiane Martins, Instituto de pesquisas energéticas e nucleares

CETER-IPEN (Centro de Tecnologia das Radiações)

Daniel Perez Vieira, Instituição de pesquisas energéticas e nucleares

CEBIO( Centro de Biotecnologia)

Luma Ramirez de Carvalho, Instituição de pesquisas energéticas e nucleares

CEBIO( Centro de Biotecnologia)

Angélica Bueno Barbezan, Instituição de pesquisas energéticas e nucleares

CR( Centro de Radiofarmácia)

Anna Lucia Casañas Haasis Villavicencio, Instituição de pesquisas energéticas e nucleares

CETER( Centro de Tecnologia das Radiações)

References

SATIM, M. Food Irradiation a Guidebook, Technomic Publishing Co.Inc, Lancester, PA, 1993.

FARKAS, J.; EHLERMANN, D.A.E. “Food irradiation”, In:Encyclopedia of Food Safety. Foods, Materials, Techn Mohacsi-Farkasologies and Risks, v.3, p. 178-186, 2014.

VARIYAR, P.S.; CHATTERJEE, S.; SAJILATA, M.G.; SINGHAL, R.S.; SHARMA,A. Natural existence of 2-alkylcyclobutanones. Journal of Agricultural and Food Chemistry, v. 56, p. 11817– 11823, 2008.

DELINCÉE, H.; POOL-ZABEL, B.L. Genotoxic properties of 2-dodecylcyclobutanone, a compound formed on irradiation of food containing fat. Radiation Physics and Chemistry, V.52, p.39–42, 1998.

LETELLIE, P.R.; NAWAR, W.W. 2-Alkylcyclobutanones from radiolysis of triglycerides. Lipids, v.7, p. 75–76, 1972.

CHAN, W.C.; YE, Y. LEUNGE,E.M.K. Rapid identification of γ-irradiated food by direct solvent extraction and liquid chromatography-tandem mass spectrometric analysis of 2-Dodecylcyclobutanone: Application in surveillance of irradiated food. Food chemistry, v.161, p. 312-316, 2014.

STEVENSON, M.H. Validation of the cyclobutanone protocol for detection of irradiated lipid containing foods by interlaboratory trial. In: MC MURRAY , C.H.; STEWART, E.M.; GRAY,R; PEARCE,J., (Eds.), Detection Methods for Irradiated Foods – Current Status. Royal Society of Chemistry, Cambridge, UK, p. 269–284, 1996.

NDIAYE B.; JAMET, G.; MIESCH, M.; HASSELMANN, C.; MARCHIONI, E. 2-Alkylcyclobutanones as markers for irradiated foodstuffs II. The CEN (European Committee for Standardization) method: field of application andlimit of utilization, Radiation Physics and Chemistry, v. 55, p. 437– 445,1999.

BURNOUF, D.; DELINCÉE, H.; HARTWING, A.; MARCHIONI, E.; MIESCH,M.;RAUL F.;WERNER,D.Etude toxicologique transfrontalière destinée à évaluer le risqué encourulors de la consummation d’aliments grasionisés – Toxikol-ogische Untersuchungzur Risikoberwertungbeim Verzehr von bestrahltenfetthaltigen Lebensmitteln – Eine französisch-deutsche Studieim Grenzraum Oberrhein, Rapport final d’étude Interreg II, project No 3171, A summary of the report is available on Internet at: http://www.iaea.org/programmes/rifa/icgfi/documents/summary-press.pdf ,2002.

RAUL,F.; GOSSÉ F.; DELINCÉE, H.; HARTWING, A.; MARCHIONI, E.; MIESCH, M.;WERNER,D.; BURNOUF, D. Food-borne radiolytic compounds (2-alkylcyclobutanones) maypromote experimental colon carcinogenesis. Nutrition and Cancer, v.44, p. 88– 191, 2002.

YAMAKAGE,K.; SUI,H.; OHTA, R.;TYOIZUMI, T.; KAWAKAMI, K.;MATSUMOTO, H.; TAKAHASHI ,T.;SASAKI, K.;IKEZUME, M.;NEGISHI, S.N.; IZUME,K.; TODORIKI,S.; TAKAHASHI, K.; FURUTA, M. Genotoxic potencial and in vitro tumour-promoting potential of 2-dodecylcyclobutanone and 2-tetradecylcyclobutanone, two radiolytic products of fatty acids. Mutation Research, V.770, p. 94-104, 2014.

GADGIL, P.; HACHMEISTER, K.; SMITH, J.S.; KROPF, D.H. 2-Alkylcyclobutanones as Irradiation Dose Indicators in Irradiated Ground Beef Patties. Journal of Agricultural and Food Chemistry, v.50, p. 5746-5750, 2002.

WANG, H.; MALEKY, F. Effects of cocoa butter triacylglycerides and minor compounds on oil migration. Food Research International, v.106, p. 213-224, 2018.

BRYCE, S.M.; BEMIS, J.C.; AVLASEVICH, S. L.; DERTINGER, S.D. In vitro micronucleus assay scored by flow cytometry provides a comprehensive evaluation of cytogenetic damage and cytotoxicity, Mutation Research, v.630, p.78-91, 2007.

HORVATOVICH, P.; RAUL, F.; MIESCH, M.; BURNOUF, D.; DELINCÉE, H.; HARTWING, A.; WERNER, D.; MARCHIONI, E. Detection of 2-alkylcyclobutanones, markers for irradiated foods, in adipose tissues of animals fed with these substances. Journal of Food Protection, v.65, p. 1610–1613, 2002.

SONG, B.S.; KIM, Y.; JIN, Y.B.; KANG, I.J.; KIM,, K.S.; PARK, J.K.; KIM, J.K.; PARK, H.Y.; JEONG, S.H. Toxicological evaluation of 2-Dodecylcyclobutanone, a unic radiolitic compound of palmitic acid, Food and Chemical Toxicology, v.121, p. 639-647, 2018.

CLIET, I.; FOURNIER, E.; MELCION, C.; CORDIER, A. In vivo micronucleus test using mouse hepatocytes, Mutation Research, v.216, p. 321-326, 1989.

Downloads

Published

2021-04-30

How to Cite

Martins, R., Perez Vieira, D., Ramirez de Carvalho, L., Bueno Barbezan, A., & Villavicencio, A. L. C. H. (2021). In vivo genotoxicity of 2-alkylcyclobutanones in liver cells from rats fed with irradiated cocoa butter using flow citometry. Brazilian Journal of Radiation Sciences, 9(1A). https://doi.org/10.15392/bjrs.v9i1A.1534

Issue

Section

The Meeting on Nuclear Applications (ENAN) 2019

Most read articles by the same author(s)