Electron beam irradiation of combined pharmaceuticals: propranonol and fluoxetine and related ecotoxicity

Authors

  • Nathalia Fonseca Boiani Nuclear and Energy Research Institute - University of Sao Paulo
  • Vanessa Silva Garcia Granadeiro Nuclear and Energy Research Institute - University of Sao Paulo
  • Sueli Ivone Borrely Nuclear and Energy Research Institute - University of Sao Paulo

DOI:

https://doi.org/10.15392/bjrs.v9i1A.1575

Keywords:

Fluoxetine, Mixture, Pharmaceuticals, Propranolol

Abstract

There are serious evidences that justify the search for treatment technologies or processes combination for the improvement of decomposition for dozens of pharmaceuticals in wastewaters. Electron beam irradiation may play an important role in this scenario and relatively low doses have been reported for such purposes. The aim of the present study was to evaluate the toxic response of the crustacean Daphnia similis exposed to individual and combined pharmaceuticals, before and after electron beam irradiation treatment. Several experimental trials of an acute immobilization test were performed with a mixture of pharmaceuticals composed of fluoxetine hydrochloride (Prozac®), and propranolol. Single pharmaceuticals were first tested separately. Toxicity of binary mixture was then assessed using five concentrations and five percentages of each substance in the mixture (0, 25, 50, 75, and 100%). Acute EC50% values ranged from 5.0 to 7.4 for fluoxetine and from 11.3 to 13.7 for propranolol. In mixture, values ranged from 6.4 to 9.8. Fluoxetine was more toxic than propranolol for D.similis. The different pharmaceuticals concentrations employed in a mixture showed no difference in toxicity values. When electron beam irradiation was applied, approximately 80% of acute effects were reduced at 5 kGy, and the mixture containing a higher percentage of fluoxetine, also showed a greater reduction of toxicity.

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References

FENT K., WESTON A.A., CAMINADA D., Ecotoxicology of human pharmaceuticals. Aquat Toxicol, 76:122–159, 2006.

CALAMARI D., ZUCCATO E., CASTIGLIONI S., BAGNATI R., FANELLI R., Strategic survey of therapeutic drugs in the rivers Po and Lambro in northern Italy. Environ Sci Technol, 37:1241–1248, 2003.

SANTOS L.H., ARAÚJO A.N., FACHINI A., PENA A., DELERUE-MATOS C., MONTENEGRO M.C., Ecotoxicological aspects related to the presence of pharmaceuticals in the aquatic environment. J Hazard Mater, 175:45–95, 2010.

FABBRI E. AND FRANZELLITTI S., Human pharmaceuticals in the marine environment: Focus on exposure and biological effects in animal species. Environ Toxicol Chem, 35:799–812, 2016.

GLASSMEYER ST, KOLPIN DW, FURLONG ET, FOCAZIO MJ., Environmental presence and persistence of pharmaceuticals: an overview. In: Aga DS (ed) Fate of Pharmaceuticals in the Environment and in Water Treatment Systems. Taylor & Francis, Boca Raton, FL, 2008.

BARATA C., BAIRD D.J., NOGUEIRA A.J., SOARES A.M.V.M., RIVA M.C., Toxicity of binary mixtures of metals and pyrethroid insecticides to Daphnia magna Straus. Implications for multi-substance risks assessment. Aquat Toxicol, 78:1–14, 2006.

RUDD M.A., ANKLEY G.T., BOXALL A.B.A., BROOKS B.W., International scientists’ research priorities for pharmaceuticals and personal care products in the environment. Integr Environ Assess Manag, 10:576–587, 2014.

TERNES T. A., STÜBE, J., HERRMANN N., MCDOWELL D., RIED A., KAMPMANN M., TEISE, B., Ozonation: a tool for removal of pharmaceuticals, contrast media and musk fragrances from wasterwater?. Water Research. 37(8):1976-1982, 2003.

GODOY A.A., KUMMROW F., PAMPLIN P.A.Z., Occurrence, ecotoxicological effects and risk assessment of antihypertensive pharmaceutical residues in the aquatic environment - A review. Chemosphere, 138:281–29, 2015.

MASZKOWSKA J., STOLTE S., KUMIRSKA J., LUKASZEWICZ P., MIODUSZEWSKA K., PUCKOWSKI A., CABAN M., WAGIL M., STEPNOWSKI P., BIALKBIELINŃSKA A., Beta-blockers in the environment: Part II. Ecotoxicity study. Sci Total Environ, 493:1122–1126, 2014.

FONG P.P., FORD A.T., The biological effects of antidepressants on the molluscs and crustaceans: a review. Aquat Toxicol, 151:4–13, 2014.

METCALFE C.D., CHU S., JUDT C., LI H., OAKES K.D., SERVOS M.R., ANDREWS D.M., Antidepressants and their metabolites in municipal wastewater, and downstream exposure in an urban watershed. Environ Toxicol Chem, 29:79–89, 2010.

DEBLONDE T., COSSU-LEGUILL C., HARTEMANN P., Emerging pollutants in wastewater: A review of the literature. International Journal of Hygiene and Environmental Health, 214, 442-448, 2011.

BRINGOLF R.B., HELTSLEY R.M., NEWTON T.J., EADS C.B., FRALEY S.J., SHEA D., COPE W.G., Environmental occurrence and reproductive effects of the pharmaceutical fluoxetine in native freshwater mussels. Environ Toxicol Chem, 29:1311–1318, 2010.

DAUGHTON C.G., BROOKS B.W., Active pharmaceuticals ingredients and aquatic organisms. In: Beyer WN, Meador J (eds) Environmental contaminants in biota: interpreting tissue concentrations. Taylor and Francis, Boca Raton, 2nd edn.,287–347, 2011.

FRANZELLITTI S., BURATTI S., CAPOLUPO M., DU B., HADDAD S.P., CHAMBLISS C.K., BROOKS B.W., FABBRI E., An exploratory investigation of various modes of action and potential adverse outcomes of fluoxetine in marine mussels. Aquat Toxicol, 151:14–26 (2014).

HADAYATIRAD M., NEMATOLLAHI M.A., FORSATKAR M.N., Prozac impacts lateralization of aggression in male Siamese fighting fish. Ecotox and Env Saf, 140: 84-88, 2017.

WEINBERGER J., KLAPER R., Environmental concentrations of the selective serotonin reuptake inhibitor fluoxetine impact specific behaviors involved in reproduction, feeding and predator avoidance in the fish Pimephales promelas (fathead minnow). Aquat Tox, 151:77–83, 2014.

SANTOS D.R.A., GARCIA V.S.G., VILARRUBIA A.C.F., BORRELY S.I., Acute toxicity assessment of fluoxetine hydrochloride (Prozac®) when submitted to electron beam irradiation. Internat Conf on Dev and Applic of Nuc Tech, 2011.

SILVA V.H.O., BATISTA A.P.S., TEIXEIRA A.C.S.C., BORRELY S.I., Degradation and acute toxicity removal of the antidepressant Fluoxetine (Prozac) in aqueous systems by electron beam irradiation. Env Sci and Pollut Res, 2016.

TOMINAGA F.K., BATISTA A.P., TEIXEIRA A.C.S.C., BORRELY S.I., Degradation of diclofenac by electron beam irradiation: Toxicity removal, by-products identification and effect of another pharmaceutical compound. J of Env Chem Eng, 6(4):4605-4611, 2018.

HAMILTON M.A., RUSSO R.C., THURSTON R.V., Trimmed Spearman Karber Method for estimating median lethal concentrations on toxicity bioassays. Envir Sci & Tech, 11(7):714–719, 1977.

VARANO V., FABBRI E., PATERIS A., Assessing the environmental hazard of individual and combined pharmaceuticals: acute and chronic toxicity of fluoxetine and propranolol in the crustacean Daphnia magna. Ecot, 26(6):711-728, 2017.

TIERNEY A.J., Structure and function of invertebrate 5-HT receptors: a review. Comp Biochem Physiol A Mol Integr Physiol, 128:791–804, 2001.

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Published

2021-04-30

How to Cite

Boiani, N. F., Granadeiro, V. S. G., & Borrely, S. I. (2021). Electron beam irradiation of combined pharmaceuticals: propranonol and fluoxetine and related ecotoxicity. Brazilian Journal of Radiation Sciences, 9(1A). https://doi.org/10.15392/bjrs.v9i1A.1575

Issue

Section

The Meeting on Nuclear Applications (ENAN) 2019

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