Study of the radiomodifier effect of Pityrocarpa moniliformis extract

Authors

  • João Victor Torres de Moraes Universidade Federal de Pernambuco
  • Ricardo Luiz Calazans Luna Filho Universidade Federal de Pernambuco
  • Williams Nascimento de Siqueira Universidade Federal de Pernambuco
  • Hianna Arely Milca Fagundes Silva Universidade Federal de Pernambuco
  • Dewson Rocha Pereira Universidade Federal de Pernambuco
  • Ana Maria Mendonça de Albuquerque Melo Universidade Federal de Pernambuco

DOI:

https://doi.org/10.15392/bjrs.v7i2A.760

Keywords:

Biomphalaria glabrata, Pityrocarpa moniliformis, Radiation

Abstract

Ionizing radiation has been applied in several areas of knowledge, among them the study of the radiomodifier activity of natural substances. These substances can modify the cellular response to the damage induced by the radiation. Therefore, this work aimed to evaluate the radiomodifier action of Pityrocarpa moniliformis extract on Biomphalaria glabrata embryos exposed to 60Co gamma radiation. Initially, toxicity tests were performed on the extract against the B. glabrata embryos for the choice of concentration that did not cause death and embryonic malformation. Then, the antioxidant activity of the P. moniliformis extract with flavonoids and phenolic compounds was evaluated by means of the ABTS method. To evaluate the radiomodifier activity of the extract, embryos were selected in the blastula stage and irradiated with 7.5 Gy in a 60Co source (gammacell-Co60). Then, the embryos were exposed for 24 h to the extract of P. moniliformis at a concentration of 250 μg/mL. The results showed that the extract of P. moniliformis presents flavonoids and enzymatic inhibition by ABTS, which demonstrates the presence of antioxidant compounds. However, the tests of the radiomodifier activity did not present radioprotective effect for embryos exposed to ionizing radiation.

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Author Biographies

João Victor Torres de Moraes, Universidade Federal de Pernambuco

Departamento de Biofísica e Radiobiologia

Ricardo Luiz Calazans Luna Filho, Universidade Federal de Pernambuco

Departamento de Biofísica e Radiobiologia

Williams Nascimento de Siqueira, Universidade Federal de Pernambuco

Departamento de Biofísica e Radiobiologia

Hianna Arely Milca Fagundes Silva, Universidade Federal de Pernambuco

Departamento de Biofísica e Radiobiologia

Dewson Rocha Pereira, Universidade Federal de Pernambuco

Departamento de Biofísica e Radiobiologia

Ana Maria Mendonça de Albuquerque Melo, Universidade Federal de Pernambuco

Departamento de Biofísica e Radiobiologia

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Published

2019-02-25

How to Cite

Moraes, J. V. T. de, Luna Filho, R. L. C., Siqueira, W. N. de, Silva, H. A. M. F., Pereira, D. R., & Melo, A. M. M. de A. (2019). Study of the radiomodifier effect of Pityrocarpa moniliformis extract. Brazilian Journal of Radiation Sciences, 7(2A (Suppl.). https://doi.org/10.15392/bjrs.v7i2A.760