DNA damage Evaluation in a Nursing Team Occupationally Exposed to Ionizing Radiation

Iranez Bortolotto; Ana Paula S. Brum; Larissa M. de Souza; Cristiano Trindade; Temenouga N. Guecheva; Fabiano M Luiz; Ana Ligia L de Paula-Ramos; Angelica R. Consiglio

doi:10.15392/bjrs.v3i1.82

Authors

Iranez Bortolotto UFRGS_Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Depto de Biofísica e PPG_Enfermagem
Ana Paula S. Brum UFRGS_Universidade Federal do Rio Grande do Sul, PPG_Enfermagem
Larissa M. de Souza PPG Biologia Molecular e Celular
Cristiano Trindade PPG Biologia Molecular e Genética
Temenouga N. Guecheva UFRGS_Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Depto de Biofísica
Fabiano M Luiz UFRGS_Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Depto de Biofísica
Ana Ligia L de Paula-Ramos UFRGS_Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Depto de Biofísica
Angelica R. Consiglio UFRGS_Universidade Federal do Rio Grande do Sul, Instituto de Biociências, Depto de Biofísica e PPG_Enfermagem

DOI:

https://doi.org/10.15392/bjrs.v3i1.82

Abstract

Purpose: To evaluate DNA damage in lymphocytes and cognitive deficits in a nursing team occupationally exposed to ionizing radiation in a university hospital in southern Brazil. Method: Cross-sectional study, case-control design, included 79 women working in a nursing team in Hemodynamics, Radiology, Ambulatory and Midwifery Units. They were classified in two groups: 1_Exposed to ionizing radiation, n=38 (Hemodynamics and Radiology Units) and 2_Unexposed to ionizing radiation, n=41 (Ambulatory and Midwifery Unit). Blood was collected and DNA damage in lymphocytes was analyzed using the comet assay and micronucleus test (MN). The cells were classified according to the damage frequency (DF) and index (DI) based on the comet tail size. Cognition was also evaluated according to the scores obtained in Stroop test, the digit and word span. Results: No difference was found for the variables DI, DF, MN, Stroop_word, Stroop_color, Stroop_color/word between the groups. However, when the groups were analyzed independently of their exposure condition, the damage index in the Hemodynamics was lower than in all other Units. The damage frequency in Midwifery Unit was higher than in Ambulatory and Hemodynamics. The frequency of micronucleus was not different among the employees from the four Units. Conclusions: DNA damage in lymphocytes did not differ between nursing teams occupationally exposed or not exposed to ionizing radiation. However, higher damage in a non exposed group may indicate other risks to the healthcare team.

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References

ALVAREZ, J.A.; EMORY, E. Executive Function and the Frontal Lobes: A Meta-Analytic Review. Neuro Rev, 16 No. 1, 2006.

ANDERSEN, S. L.; TEICHER, M.H. Delayed effects of early stress on hippocampal development Neuropsychopharmacology, 29, 1988 – 1993, 2004.

ANDRES-MACH, M.; ROLA, R.; FIKE, J. Radiation effects on neural precursor cells in the dentate gyrus Cell Tissue Res, 331, 251 – 262, 2008.

BEIR, VII. 2006 Health risks from exposure to low levels of ionizing radiation. BEIR VII phase, 2.

BOURAOUI, S.; MOUGOU, S.; DRIRA, A.; TABKA, F.; BOUALI, N.; MRIZEK, N.; ELGHEZAL, H.; SAAD, A. A cytogenetic approach to the effects of low levels of ionizing radiation (IR) on the exposed tunisian hospital workers. Int J Occup Med Environ Health, 26, 144 – 154, 2013.

BRENDLER-SCHWAAB, S.; HARTMANN, A.; PFUHLER, S.; SPEIT, G. The in vivo comet assay: use and status in genotoxicity testing. Mutagenesis, 20, 245 – 54, 2005.

BRENNER, D.J. Cancer risks attributable to low doses of ionizing radiation: assessing what we really know. Proceedings of the National Academy of Science of the United States of America. 100, 13761 – 13766, 2003.

BROOKS, A.; LAWRENCE, D. Radiation Dosimetry and Risk in the Practice of Nuclear Medicine. Advances in Radiation Biology: Effect on Nuclear Medicine, 44, 179 – 186, 2014.

CACERES, L. Hippocampal-related memory deficits and histological damage induced by neonatal ionizing radiation exposure. Role of oxidative status. Brain Res, 1312, 67 – 78, 2009.

COLLINS, A.R.; OSCOZ, A.A.; BRUNBORG, G.; GAIVÃO, I.; GIOVANNELLI, L.; KRUSZEWSKI, M.; SMITH, C.C.; STETINA, R. The comet assay: topical issues. Mutagenesis, 23, 143 – 51, 2008.

COLLINS, A.R. The cometa assay for DNA damage and repair: principles, applications and limitations. Mol Biotechnol, 26, 249 – 261, 2004.

DAINIAK, N. Hematologic consequences of exposure to ionizing radiation. Exp Hematol, 30, 513–528, 2002.

DOETSCH, F.; GARCÍA-VERDUGO, J.M.; ALVAREZ-BUYLLA, A. Regeneration of a germinal layer in the adult mammalian brain. Proc Natl Acad Sci USA, 96, 11619 – 11624, 1999.

ERIKSSON, P.S. et al. Neurogenesis in the adult human hippocampus. Nat Med, 4, 1313 – 1317, 1998.

FAIRBAIRN, D.W.; OLIVE, P.L.; O'NEILL, K.L. The comet assay: a comprehensive review. Mutat Res, 339, 37 – 59, 1995.

FENECH, M. Cytokinesis-block micronucleus cytome assay. Nat Protoc, 2, 1084 – 1104, 2007.

FENECH, M. Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells. Mutagenesis, 26, 125 – 132, 2011.

FENECH, M. Chromosomal biomarkers of genomic instability relevant to cancer. Drug Discov Today, 7, 1128 – 1137, 2002.

FENECH, M.; CHANG, W.P.; KIRSCH-VOLDERS, M.; HOLLAND, N.; BONASSI, S.; ZEIGER, E. HUMAN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat Res, 534, 65 – 75.2003.

FENECH, M.; MORLEY, A.A. Measurement of micronuclei in lymphocytes. Mutat Res, 147, 29 – 36.

HALL, E.J. Radiobiology for the radiologist. 1985.

LIAO, W.; MCNUTT, M.A.; ZHU, W.G. The comet assay: a sensitive method for detecting DNA damage in individual cells. Methods, 48, 46 – 53, 2009.

MACLEOD, C.M. Half a century of research on the Stroop effect: an integrative review. Psychological Bulletin, 109, 163 – 203, 1991.

MALUF, S.W.; PASSOS, D.F.; BACELAR, A.; SPEIT, G.; ERDTMANN, B. Assessment of DNA damage in lymphocytes of workers exposed to X-radiation using the micronucleus test and the comet assay. Environ Mol Mutagen, 38, 311 – 315, 2001.

MARTÍNEZ, A.; COLEMAN, M.; ROMERO-TALAMÁS, C.A.; FRÍAS, S. An assessment of immediate DNA damage to occupationally exposed workers to low dose ionizing radiation by using the comet assay. Rev Invest Clin, 62, 23 – 30, 2010.

MCEWEN, B.S. Allostasis, allostatic load, and the aging nervous system: role of excitatory amino acids and excitotoxicity. Neurochem Res, 25, 1219 – 1231, 2008.

MONJE, M.L.; MIZUMATSU, S.; FIKE, J.R.; PALMER, T.D. Irradiation induces neural precursor-cell dysfunction. Nat Med, 8, 955 – 962, 2002.

MOORE, P.V.; MOORE, R.L. Fundamentals of Occupational and Environmental Health Nursing. 2014.

NADIN, S.B.; VARGAS-ROIG, L.M.; CIOCCA, D.R. A silver staining method for single-cell gel assay. J Histochem Cytochem, 1, 180 – 93, 2011.

PALAZZO, R.P.; MALUF, S.W. Técnica de micronúcleos com bloqueio da citocinese celular. Citogenética Humana, 180 – 193, 2011.

PANAGIOTAKOS, G. Long-term impact of radiation on the stem cell and oligodendrocyte precursors in the brain. PLoS One, 2, 588, 2007.

PRISE, K.M.; SARAN, A. Concise review: stem cell effects in radiation risk. Stem Cells, 29, 1315 – 1321, 2011.

PRUESSNER, J.C.; DEDOVIC, K.; PRUESSNER, M.; LORD, C.; BUSS, C.; COLLINS, L. Stress regulation in the central nervous system: evidence from structural and functional neuroimaging studies in human populations. Psychoneuroendocrinology, 35, 179 – 191, 2010.

REUTER, S.; GUPTA, S.C.; CHATURVEDI, M.M.; AGGARWAL, B.B. Oxidative stress, inflammation, and cancer: How are they linked? Free Radic Biol Med, 49, 1603 – 1616, 2010.

ROLA, R.; RABER, J.; RIZK, A.; OTSUKA, S.; VANDENBERG, S.R.; MORHARDT, D.R.; FIKE, J.R. Radiation-induced impairment of hippocampal neurogenesis is associated with cognitive deficits in young mice. Exp Neurol, 188, 316 – 330, 2004.

ROPOLO, M.; BALIA, C.; ROGGIERI, P.; LODI, V.; NUCCI, M.C.; VIOLANTE, F.S.; SILINGARDI, P.; COLACCI, A.; BOLOGNESI, C. The micronucleus assay as a biological dosimeter in hospital workers exposed to low doses of ionizing radiation. Mutat Res, 747, 7 – 13, 2012.

SINGH, N.P.; MCCOY, M.T.; TICE, R.R.; SCHNEIDER, E.L. A simple technique for quantitation of low leves of DNA damange in individual cells. Exp Cell Res, 175, 184 – 191, 1988.

STRAUSS, E.; SHERMAN, E.M.S.; SPREEN, O.A. Compedium of neuropsychological tests. 2006.

TADA, E.; PARENT, J.M.; LOWENSTEIN, D.H.; FIKE, J.R. X-irradiation causes a prolonged reduction in cell proliferation in the dentate gyrus of adult rats. Neuroscience, 99, 33 – 41, 2000.

UNSCEAR (United Nations Scientific Committee on Effects of Atomic Radiation). Sources and effects of ionizing radiation. 2008.