Evaluations of the possible mutagenic and genotoxic effects of 2-ACBs: by-products generated from irradiated foods

Angélica Bueno Barbezan; Ana Carolina de Araújo Bispo; Bruno Melo Mendes; Daniel Perez Vieira; Luma Ramirez de Carvalho; Glaucia Maria Santelli; Anna Lúcia Casaas Haasis Villavicencio

doi:10.15392/2319-0612.2024.2600

Autores/as

Angélica Bueno Barbezan Instituto de Pesquisas Energéticas e Nucleares - IPEN , Instituto de Pesquisas Energéticas e Nucleares https://orcid.org/0000-0001-7615-9091 (no autenticado)
Ana Carolina de Araújo Bispo Laboratory of Genotoxicity in Microorganisms - (LGM) of the Center for the Development of Nuclear Technology (CDTN) , Universidade Federal de Minas Gerais
Bruno Melo Mendes Laboratory of Genotoxicity in Microorganisms - (LGM) of the Center for the Development of Nuclear Technology (CDTN) , Universidade Federal de Minas Gerais
Daniel Perez Vieira IPEN , Instituto de Pesquisas Energéticas e Nucleares
Luma Ramirez de Carvalho IPEN , Instituto de Pesquisas Energéticas e Nucleares
Glaucia Maria Santelli ICB/USP , Universidade de São Paulo
Anna Lúcia Casaas Haasis Villavicencio IPEN , Instituto de Pesquisas Energéticas e Nucleares

DOI:

https://doi.org/10.15392/2319-0612.2024.2600

Palabras clave:

Alimento Irradiado, 2-Alquilciclobutanonas (2-ACBs), 2-Dodecilciclobutanonas (2-dDCBs), 2-Tetradecilciclobutanonas (2-tDCBs), Prueba de Ames, Ensayo de Micronúcleos.

Resumen

Este estudio investiga los posibles efectos mutagénicos y genotóxicos de los subproductos 2-Alquilciclobutanonas (2-ACBs), formados en alimentos irradiados. Las 2-ACBs son compuestos derivados de la irradiación de alimentos que contienen grasa, con reconocido potencial genotóxico. La investigación se centró en los compuestos 2-dodecilciclobutanona (2-dDCB) y 2-tetradecilciclobutanona (2-tDCB), evaluando su mutagenicidad mediante el ensayo de micronúcleos en líneas celulares hepáticas (HepG2, BRL3A y HTC) y genotoxicidad a través del ensayo de Ames utilizando cinco cepas bacterianas (TA-98, TA-100, TA-1535, TA-1537 y WP2uvrA). Los resultados del ensayo de Ames indicaron que 2-dDCB y 2-tDCB no aumentaron significativamente las tasas de reversión mutagénica, mientras que los ensayos de micronúcleos mostraron ausencia de daños genotóxicos en las líneas celulares analizadas. Se concluye que, en las concentraciones evaluadas, los compuestos 2-dDCB y 2-tDCB no presentan potencial mutagénico ni genotóxico, lo que corrobora la seguridad de los alimentos irradiados. No obstante, se recomienda la realización de investigaciones adicionales para evaluar los efectos a largo plazo y bajo diferentes condiciones de irradiación

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Biografía del autor/a

Angélica Bueno Barbezan, Instituto de Pesquisas Energéticas e Nucleares - IPEN, Instituto de Pesquisas Energéticas e Nucleares

I hold a Bachelor's degree in Biomedicine from the Centro Universitário das Faculdades Metropolitanas Unidas (2007), with specializations in clinical analysis, imaging, and pharmacology. Subsequently, I joined the Institute of Energy and Nuclear Research at the University of São Paulo (IPEN/USP), where I earned my Master’s degree in 2012. My Master’s project focused on comparing the labeling of phosphonates, specifically MDP, EDTMP, and Clodronate, with potential therapeutic applications for patients with bone metastases. This initial experience sparked my interest in the therapeutic applications of nuclear biomedicine, motivating me to delve deeper into research.

Furthering my commitment to research, I pursued and completed my Ph.D. at the same institution in 2017, concentrating on in vitro studies of the genotoxicity and cytotoxicity in hepatic cells due to the formation of 2-Alkylcyclobutanones from the irradiation of fat-containing foods. This work provided me with a deeper understanding of the biological effects of radiation and reinforced my dedication to developing safer and more effective therapies.

Currently, I am part of a specialized team in nanobrachytherapy, focusing on the research and development of radioactive gold-198 nanoparticles for prostate cancer therapy. My work involves conducting biological tests, including both in vitro and in vivo studies, with the aim of contributing to significant advancements in cancer treatment, providing new therapeutic options that can improve patient outcomes and quality of life.

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