Evaluations of the possible mutagenic and genotoxic effects of 2-ACBs: by-products generated from irradiated foods

Angélica Bueno Barbezan; Ana Carolina de Araújo Bispo; Bruno Melo Mendes; Daniel Perez Vieira; Luma Ramirez de Carvalho; Glaucia Maria Santelli; Anna Lúcia Casaas Haasis Villavicencio

doi:10.15392/2319-0612.2024.2600

Authors

Angélica Bueno Barbezan Instituto de Pesquisas Energéticas e Nucleares - IPEN , Nuclear Energy Research Institute https://orcid.org/0000-0001-7615-9091 (unauthenticated)
Ana Carolina de Araújo Bispo Laboratory of Genotoxicity in Microorganisms - (LGM) of the Center for the Development of Nuclear Technology (CDTN) , Federal University of Minas Gerais
Bruno Melo Mendes Laboratory of Genotoxicity in Microorganisms - (LGM) of the Center for the Development of Nuclear Technology (CDTN) , Federal University of Minas Gerais
Daniel Perez Vieira IPEN , Nuclear Energy Research Institute
Luma Ramirez de Carvalho IPEN , Nuclear Energy Research Institute
Glaucia Maria Machado-Santelli ICB/USP , University of São Paulo
Anna Lúcia Casaas Haasis Villavicencio IPEN , Nuclear Energy Research Institute

DOI:

https://doi.org/10.15392/2319-0612.2024.2600

Keywords:

Food Irradiation, Alkylcyclobutanones (2-ACBs), 2-dodecylcyclobutanone (2-dDCB), 2-tetradecylcyclobutanone (2-tDCB), Ames Test, Micronucleus Assay

Abstract

This study investigates the potential mutagenic and genotoxic effects of 2-Alkylcyclobutanones (2-ACBs), by-products formed in irradiated foods. 2-ACBs are compounds derived from the irradiation of fat-containing foods, with recognized genotoxic potential. The research focused on the compounds 2-dodecylcyclobutanone (2-dDCB) and 2-tetradecylcyclobutanone (2-tDCB), evaluating their mutagenicity through the micronucleus assay in hepatic cell lines (HepG2, BRL3A, and HTC) and genotoxicity through the Ames test using five bacterial strains (TA-98, TA-100, TA-1535, TA-1537, and WP2uvrA). Results from the Ames test indicated that 2-dDCB and 2-tDCB did not significantly increase mutagenic reversion rates, while the micronucleus assays showed no genotoxic damage in the tested cell lines. It is concluded that, at the evaluated concentrations, the compounds 2-dDCB and 2-tDCB do not exhibit mutagenic or genotoxic potential, supporting the safety of irradiated foods. However, further research is recommended to assess long-term effects and different irradiation conditions.

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Author Biography

Angélica Bueno Barbezan, Instituto de Pesquisas Energéticas e Nucleares - IPEN, Nuclear Energy Research Institute

I hold a Bachelor's degree in Biomedicine from the Centro Universitário das Faculdades Metropolitanas Unidas (2007), with specializations in clinical analysis, imaging, and pharmacology. Subsequently, I joined the Institute of Energy and Nuclear Research at the University of São Paulo (IPEN/USP), where I earned my Master’s degree in 2012. My Master’s project focused on comparing the labeling of phosphonates, specifically MDP, EDTMP, and Clodronate, with potential therapeutic applications for patients with bone metastases. This initial experience sparked my interest in the therapeutic applications of nuclear biomedicine, motivating me to delve deeper into research.

Furthering my commitment to research, I pursued and completed my Ph.D. at the same institution in 2017, concentrating on in vitro studies of the genotoxicity and cytotoxicity in hepatic cells due to the formation of 2-Alkylcyclobutanones from the irradiation of fat-containing foods. This work provided me with a deeper understanding of the biological effects of radiation and reinforced my dedication to developing safer and more effective therapies.

Currently, I am part of a specialized team in nanobrachytherapy, focusing on the research and development of radioactive gold-198 nanoparticles for prostate cancer therapy. My work involves conducting biological tests, including both in vitro and in vivo studies, with the aim of contributing to significant advancements in cancer treatment, providing new therapeutic options that can improve patient outcomes and quality of life.

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