Microbeam X-ray fluorescence mapping of Cu and Fe in human prostatic carcinoma cell lines using synchrotron radiation

Authors

  • Karolynne José Rocha Nuclear Instrumentation Laboratory/Federal University of Rio de Janeiro
  • Roberta Gama Leitão Physics Institute/Stated University of Rio de Janeiro
  • Eliane Oliveira Barros Institute of Biomedical Sciences/Federal University of Rio de Janeiro
  • Maria Aparecida Oliveira Institute of Biomedical Sciences/Federal University of Rio de Janeiro
  • Catarine Gondim Canellas Physics Institute/Stated University of Rio de Janeiro
  • Marcelino José Anjos Physics Institute/Stated University of Rio de Janeiro
  • Luiz Eurico Nasciutti Institute of Biomedical Sciences/Federal University of Rio de Janeiro
  • Ricardo Tadeu Lopes Nuclear Instrumentation Laboratory/Federal University of Rio de Janeiro

DOI:

https://doi.org/10.15392/bjrs.v7i2A.608

Keywords:

X-Ray, microfluorescence, cancer

Abstract

Cancer is a worldwide public health problem and prostate cancer continues to be one of the most common fatal cancers in men. Copper plays an important role in the aetiology and growth of tumours however, whether intratumoral copper is actually elevated in prostate cancer patients has not been established. Iron, an important trace element, plays a vital function in oxygen metabolism, oxygen uptake, and electron transport in mitochondria, energy metabolism, muscle function, and hematopoiesis. The X-ray microfluorescence technique (μXRF) is a rapid and non-destructive method of elemental analysis that provides useful elemental information about samples without causing damage or requiring extra sample preparations. This study investigated the behavior of cells in spheroids of human prostate cells, tumour cell line (DU145) and normal cell line (RWPE-1), after supplementation with zinc chloride by 24 hours using synchrotron X-ray microfluorescence (μSRXRF). The measurements were performed with a standard geometry of 45º of incidence, excited by a white beam using a pixel of 25 μm and a time of 300 ms/pixel at the XRF beamline at the Synchrotron Light.

Downloads

Download data is not yet available.

References

Web Site

National Cancer Institute José Alencar Gomes da Silva. Estimate 2016: Cancer Incidence in Bra-zil. 2016. Available at: http://www.inca.gov.br/wcm/dncc/2015/estimativa-2016.pdf>.

Journal

GOUGH, Mallory et al. The E1 copper binding domain of full-length amyloid precursor protein mitigates copper-induced growth inhibition in brain metastatic prostate cancer DU145 cells. Bio-chemical And Biophysical Research Communications, v. 453, p. 741-747, 2014.

Journal

DENOYER, Delphine et al. Heterogeneous copper concentrations in cancerous human prostate tis-sues. The Prostate, v. 75, p. 1510-1517, 2015.

Journal

DENOYER, Delphine et al. Copper as a target for prostate cancer therapeutics: copper-ionophore pharmacology and altering systemic copper distribution. Oncotarget, v. 7, p. 37064-37080, 2016.

Journal

LUDWIG, Heinz et al. Iron metabolism and iron supplementation in cancer patients. Wiener Klinische Wochenschrift, v. 127, p. 907-919, 2015.

Journal

RODRIGO, Miguel Angel Merlos et al. Zinc and metallothionen in prostate cancer: A review. Journal Of Metallomics And Nanotechnologies, v. 2, p. 58-63, 2015.

Journal

COSTELLO, Leslie C.; FRANKLIN, Renty B. A comprehensive review of the role of zinc in nor-mal prostate function and metabolism; and its implications in prostate cancer. Archives Of Bio-chemistry And Biophysics, v. 611, p. 100-112, 2016.

Journal

JOHNSON, Leslie A. et al. Differential zinc accumulation and expression of human zinc transporter 1 (hZIP1) in prostate glands. Methods, v. 52, p. 316-321, 2010.

Journal

LANKOSZ, M. et al. Research in quantitative microscopic X-ray fluorescence analysis. Spectrochimica Acta Part B: Atomic Spectroscopy, v. 59, p. 1517-1521, 2004.

Journal

RAK, Margaret et al. X-ray microfluorescence (μXRF) imaging of Aspergillus nidulans cell wall mutants reveals biochemical changes due to gene deletions. Analytical And Bioanalytical Chem-istry, v. 406, p. 2809-2816, 2014.

Journal

ROCHA, K.M.J et al. H Zinc distribution in human prostate carcinoma cell line using synchrotron X-ray microfluorescence. X-Ray Spectrometry, v. 46, p. 403-411, 2017.

Journal

XUE, Dong et al. Decreased expression of ferroportin in prostate cancer. Oncology Letters, v. 10, p. 913-916, 2015.

Downloads

Published

2019-02-19

How to Cite

Rocha, K. J., Leitão, R. G., Barros, E. O., Oliveira, M. A., Canellas, C. G., Anjos, M. J., Nasciutti, L. E., & Lopes, R. T. (2019). Microbeam X-ray fluorescence mapping of Cu and Fe in human prostatic carcinoma cell lines using synchrotron radiation. Brazilian Journal of Radiation Sciences, 7(2A (Suppl.). https://doi.org/10.15392/bjrs.v7i2A.608

Most read articles by the same author(s)