Microbeam X-ray fluorescence mapping of Cu and Fe in human prostatic carcinoma cell lines using synchrotron radiation

Authors

  • Karolynne José Rocha Nuclear Instrumentation Laboratory/Federal University of Rio de Janeiro
  • Roberta Gama Leitão Physics Institute/Stated University of Rio de Janeiro
  • Eliane Oliveira Barros Institute of Biomedical Sciences/Federal University of Rio de Janeiro
  • Maria Aparecida Oliveira Institute of Biomedical Sciences/Federal University of Rio de Janeiro
  • Catarine Gondim Canellas Physics Institute/Stated University of Rio de Janeiro
  • Marcelino José Anjos Physics Institute/Stated University of Rio de Janeiro
  • Luiz Eurico Nasciutti Institute of Biomedical Sciences/Federal University of Rio de Janeiro
  • Ricardo Tadeu Lopes Nuclear Instrumentation Laboratory/Federal University of Rio de Janeiro

DOI:

https://doi.org/10.15392/bjrs.v7i2A.608

Keywords:

X-Ray, microfluorescence, cancer

Abstract

Cancer is a worldwide public health problem and prostate cancer continues to be one of the most common fatal cancers in men. Copper plays an important role in the aetiology and growth of tumours however, whether intratumoral copper is actually elevated in prostate cancer patients has not been established. Iron, an important trace element, plays a vital function in oxygen metabolism, oxygen uptake, and electron transport in mitochondria, energy metabolism, muscle function, and hematopoiesis. The X-ray microfluorescence technique (μXRF) is a rapid and non-destructive method of elemental analysis that provides useful elemental information about samples without causing damage or requiring extra sample preparations. This study investigated the behavior of cells in spheroids of human prostate cells, tumour cell line (DU145) and normal cell line (RWPE-1), after supplementation with zinc chloride by 24 hours using synchrotron X-ray microfluorescence (μSRXRF). The measurements were performed with a standard geometry of 45º of incidence, excited by a white beam using a pixel of 25 μm and a time of 300 ms/pixel at the XRF beamline at the Synchrotron Light.

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References

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Published

2019-02-19

How to Cite

Microbeam X-ray fluorescence mapping of Cu and Fe in human prostatic carcinoma cell lines using synchrotron radiation. Brazilian Journal of Radiation Sciences, Rio de Janeiro, Brazil, v. 7, n. 2A (Suppl.), 2019. DOI: 10.15392/bjrs.v7i2A.608. Disponível em: https://bjrs.org.br/revista/index.php/REVISTA/article/view/608. Acesso em: 22 dec. 2024.

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